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Our prior studies using HTh74 cells in the orthotopic murine thyroid cancer model system, we have observed that the in vivo growth rates are slower compared to other thyroid cancer cell lines (84 days to achieve 100 mm3 tumors compared with 28?5 days in other ATC cell lines). It is possible that if our study had been temporally extended, the trend in tumor volume attenuation in the TXNIP-overexpre
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Our prior studies using HTh74 cells in the orthotopic murine thyroid cancer model system, we have observed that the in vivo growth rates are slower compared to other thyroid cancer cell lines (84 days to achieve 100 mm3 tumors compared with 28?5 days in other ATC cell lines). It is possible that if our study had been temporally extended, the trend in tumor volume attenuation in the TXNIP-overexpre
1
Our prior studies using HTh74 cells in the orthotopic murine thyroid cancer model system, we have observed that the in vivo growth rates are slower compared to other thyroid cancer cell lines (84 days to achieve 100 mm3 tumors compared with 28?5 days in other ATC cell lines). It is possible that if our study had been temporally extended, the trend in tumor volume attenuation in the TXNIP-overexpre
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Oscope to enhance visualization. Weekly imaging with IVIS after injection of luciferin was performed to monitor tumor establishment and growth. There were 10?1 mice per group in each experiment, and the experiment was performed two times. (A) Representative images of one mouse per group imaged by IVIS over time are shown. (B) Quantitation of the bioluminescence from one experiment is shown with av
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Gnosis and increased mortality [49,50]. The mechanism underlying this differential glucose uptake between well-differentiated PTC and ATC is not well understood. The novel finding that TXNIP expression is low in ATC is consistent with the observed FDG uptake on PET/CT in patients with ATC, supporting a critical role for TXNIP as a metabolic regulator in thyroid cancer progression. In addition to i
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Gnosis and increased mortality [49,50]. The mechanism underlying this differential glucose uptake between well-differentiated PTC and ATC is not well understood. The novel finding that TXNIP expression is low in ATC is consistent with the observed FDG uptake on PET/CT in patients with ATC, supporting a critical role for TXNIP as a metabolic regulator in thyroid cancer progression. In addition to i
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Gnosis and increased mortality [49,50]. The mechanism underlying this differential glucose uptake between well-differentiated PTC and ATC is not well understood. The novel finding that TXNIP expression is low in ATC is consistent with the observed FDG uptake on PET/CT in patients with ATC, supporting a critical role for TXNIP as a metabolic regulator in thyroid cancer progression. In addition to i
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Ts; GE Healthcare). SuperSignal West Pico Chemiluminescent substrate (Thermo Scientific) was used to detect immunoreactivity. Re-Blot Plus Mild (Millipore) was used to strip blots for purposes of reprobing with an alternate primary antibody.ImmunohistochemistryCells were trypsinized and lysed in extraction buffer (EB; 1 Triton X-100, 10 mM Tris pH 7.4, 5 mM ethylenediaminetetraacetic acid (EDTA),
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Sing the Human Genome U133 Plus 2.0 Array (Affymetrix), performed by the Gene Expression Core of the University of Colorado Denver, Anschutz Medical Campus (Aurora, CO). Gene expression profiles were normalized by robust multichip analysis (RMA), differentially expressed genes were analyzed by fold-change, using a cut-off of 2-fold, 122 and 198 genes were found to be up and down-regulated in the k