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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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Sitive family history of confirmed cardiovascular diseases considering first degree relatives was completed. Hypertension, when the patient was not taking any medication, was defined as systolic blood pressure 140 mmHg and/or diastolic blood pressure 90 mmHg and diabetes mellitus as two fasting plasma glucose levels of 126 mg/dL or higher. Patient medication was recorded in detail and categorized
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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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Citrate buffer pH 6.0. Slides were left to cool down in the antigen retrieval solution for 20 minutes. Endogenous peroxidase activity was quenched by first incubating the specimens for 5 min in 3 hydrogen peroxide (supplied by user). The specimens were then incubated with the primary antibody against Apo-J (clone sc-6420, goat polyclonal antibody, Santa Cruz Biotechnology Inc, CA) in a dilution 1
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E. 4Laboratory of Experimental Surgery and Surgery Research, Medical School, National and Kapodistrian University of Athens, 15B Ag Thoma Str, Goudi, Athens, Greece. Received: 9 March 2014 Accepted: 14 April 2014 Published: 23 April 2014 References 1. Jenne DE, Tschopp J: Clusterin: the intriguing guises of a widely expressed glycoprotein. Trends Biochem Sci 1992, 17(4):154?59. 2. de Silva HV, Stu
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E. 4Laboratory of Experimental Surgery and Surgery Research, Medical School, National and Kapodistrian University of Athens, 15B Ag Thoma Str, Goudi, Athens, Greece. Received: 9 March 2014 Accepted: 14 April 2014 Published: 23 April 2014 References 1. Jenne DE, Tschopp J: Clusterin: the intriguing guises of a widely expressed glycoprotein. Trends Biochem Sci 1992, 17(4):154?59. 2. de Silva HV, Stu
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S so much greater.Competing interests The authors declare that there is no conflict of interest associated with this manuscript. Authors' contributions RKDE, SB, SAS and HA were involved in conception of the idea and study design and data analysis. RKDE, SB, PA and EOA were involved in recruitment of participants, data collection and compilation. RKDE, SB, SAS and EOA were involved in laboratory w
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S so much greater.Competing interests The authors declare that there is no conflict of interest associated with this manuscript. Authors' contributions RKDE, SB, SAS and HA were involved in conception of the idea and study design and data analysis. RKDE, SB, PA and EOA were involved in recruitment of participants, data collection and compilation. RKDE, SB, SAS and EOA were involved in laboratory w
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Internal carotid artery endarterectomy using synthetic polytetrafluoroethylene (PTFE) patch (W.L.Gore Associates, Newark, Delaware) under general anesthesia. Shunt for maintaining brain blood flow was not used in these operations. After endarterectomy, carotid plaque was carefully placed in 10 buffered formalin for further processing. Postoperatively, patients were normally recovered and left h