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D the antinociceptive effects of viral vectors, asBased on the behavioral results, we examined the expression of the endomorphins and serine histogranin in the dorsal horn of the spinal cord seven weeks after viral vector injection. Sections were labeled for endomorphins or SHG immunoreactivity. Immunoreactive positive cells for SHG (A) and endomorphins (B) were seen in the dorsal horn at the 7th
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Ented and de novo assembly. For mapping we took the latest set of scaffolds for the Daphnia pulex genome: the 06.09.2005 version with further filtering steps as available on http://Rozenberg et al. Frontiers in Zoology (2015) 12:Page 4 ofgenome.jgi-psf.org/Dappu1 (5,191 scaffolds with the total length of 197,261,574 bp). "Daphnia pulex Genes 2010 beta 3" annotations, provided by the wFleaBase (htt
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Ented and de novo assembly. For mapping we took the latest set of scaffolds for the Daphnia pulex genome: the 06.09.2005 version with further filtering steps as available on http://Rozenberg et al. Frontiers in Zoology (2015) 12:Page 4 ofgenome.jgi-psf.org/Dappu1 (5,191 scaffolds with the total length of 197,261,574 bp). "Daphnia pulex Genes 2010 beta 3" annotations, provided by the wFleaBase (htt
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Nm.GTPase activityThe assay was performed as described [34]. Briefly, for measuring GTPase activity in the presence of ribosomal subunits/intermediates 100 nM RbgA protein was incubated withPLOS Genetics | www.plosgenetics.orgSelective reaction monitoringTo improve our quantitation accuracy in more complex samples such as the cell lysates, we developed a selective reactionAn Interaction between Rb
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Nm.GTPase activityThe assay was performed as described [34]. Briefly, for measuring GTPase activity in the presence of ribosomal subunits/intermediates 100 nM RbgA protein was incubated withPLOS Genetics | www.plosgenetics.orgSelective reaction monitoringTo improve our quantitation accuracy in more complex samples such as the cell lysates, we developed a selective reactionAn Interaction between Rb
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F characteristic degradation products when proteinase K is incubated with the spheroplasts. These results suggest that the bulk of TatE is present in the cytoplasm, as shown for TatA, which would place a potential lid in the TatE structure on the cytoplasmic side of the membrane. However, this point requires further analysis because we cannot be certain that TatE does not contain a periplasmic dom
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Me-points prior), also characteristic with the reverse effect. The impact of Identity Integration on GAF was of slightly smaller sized magnitude, but of longer persistence, with an further effect from two time-points before GAF assessment. The reverse effect was of brief persistence. There are actually most likely various factors for the observed variations in length and (a)symmetry of persistence
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Me-points prior), also characteristic with the reverse effect. The impact of Identity Integration on GAF was of slightly smaller sized magnitude, but of longer persistence, with an further effect from two time-points before GAF assessment. The reverse effect was of brief persistence. There are actually most likely various factors for the observed variations in length and (a)symmetry of persistence
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